Uso de PCR universal de hemoplasma para avaliação de cavalos altamente expostos a picadas de carrapato no Sul do Brasil

Vieira, Thállitha Samih Wischral JaymeVidotto, OdilonGuimarães, Ana Márcia SáSantos, Andrea Pires dosNascimento, Naíla Cannes doFinger, Mariane Angélica PommereningBarros-Filho, Ivan RoqueDornbusch, Peterson TrichesBiondo, Alexander WelkerVieira, Rafael Felipe da CostaMessick, Joanne Belle

Mycoplasma sp. are hemotropic bacteria of red blood cells and the causative agent of hemoplasmosis. Diagnosis of infection is reached by polymerase chain reaction (PCR) and real-time PCR (qPCR) of the 16S RNA gene of this bacteria. The aim of this study was to screen horses for Mycoplasma sp. Infection using a pan-hemoplasma SYBR green qPCR assay. A total of 198 blood samples from horses were collected, DNA extracted and pan-hemoplasma qPCR performed. All samples were negative by qPCR. To verify the presence of amplifiable DNA, PCR for glyceraldehyde-3-phosphate dehydrogenase gene was performed on all samples. This study was part of an active surveillance program, which is critical for monitoring animal health status, particularly in horses. (AU)

1. Carthorses
2. Haemobartonellosis
3. Real-time PCR
4. Cavalos de carroceiros
5. Hemobartonelose
6. PCR em tempo Real
7. Hemotropic mycoplasma
8. Micoplasma hemotrópico