Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes

Costa, Juliana de Castro Beltrão da; Freitas, Elaine Ibrahim de; Lemos, Anderson Almeida de; Rosas, Carla de Oliveira; Medeiros, Valéria de Mello; Warnken, Márcia Barbosa; aru; Marin, Victor Augustus

p. 250-258

Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes

Costa, Juliana de Castro Beltrão daFreitas, Elaine Ibrahim deLemos, Anderson Almeida deRosas, Carla de OliveiraMedeiros, Valéria de MelloWarnken, Márcia BarbosaaruMarin, Victor Augustus

This work aimed at isolating Staphylococcus spp. from minas frescal type cheese, and for this purpose a Multiplex PCR (M-PCR) was standardized for detecting the classical Staphylococcus aureus enterotoxingenes (sea, seb, sec, sed and see), using the femA gene as a positive control for S. aureus strains. Bacterial detection directly from the minas frescal type cheese and from artificially contaminated food was tested.One hundred eleven colonies (104 coagulase-positive and seven coagulase-negative) were selected for performing M-PCR assay. Thirty-four colonies (30.62%) were positive for at least one of the fiveente rotoxin genes analyzed; enterotoxins sea and seb were the most frequently detected. The study on Staphylococci coagulase-positive samples revealed that 40% of the samples showed bacterial counts above the limit established by Brazilian legislation. (AU)