Effect of Melatonin on the plasmatic and mitochondrial integrity membrane of sperm cryopreserved ram
Sá, Priscilla Niely da CostaBranco, Yndyra Nayan Teixeira Carvalho CasteloRodrigues, Viviany SousaNascimento, Isolda Márcia Rocha doBarros, Filipe NunesBranco, Marlon de Araújo CasteloBarçante, Felipe Pereira da SilvaSouza, Jose Adalmir Torres de
This study evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in plasmatic andmitochondria membrane integrity of semen ram cryopreserved. Was used seven ejaculates of six ram,whichwere pooled in pool, according to the experimental groups: Control; T1 - 1x10-7 mol / L and T2 - 1x10-9 mol / Lmelatonin. Posteriorly the diluting the samples were packaged in straws of 0.25 ml and cryopreserved. Afterthawing at 37°C for 30 seconds, the samples were analyzed for Test thermoresistance slow (TTR) andacrosomal membrane integrity. For statistical analysis we used the Duncan test (α = 0.05) for meancomparison. Treatments 1 and 2 differed statistically control for plasma membrane integrity at times 60, 120and 180min. It was concluded that supplementation 1x10-7mol / L and 1x10-9mol / L of melatonin increased theintegrity of the plasma membrane to 60,120 and 180 minutes.(AU)
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