Efeito da melatonina sobre a viabilidade de espermatozóides criopreservados de ovinos
Sá, Priscilla Niely da CostaBranco, Yndyra Nayan Teixeira Carvalho CasteloMota, Luiz Harliton Cavalcante MonteiroRodrigues, Viviany SousaSilva, Jefferson Hallison LustosaBarros, Filipe NunesBarçante, Felipe Pereira da SilvaSouza, Jose Adalmir Torres de
This study to evaluate the effect of melatonin supplementation to TRIS-GEMA diluter in sperm viabilityof semen ram cryopreserved. Was used seven ejaculates of six ram,which were pooled in pool, according to theexperimental groups: T1 - control; T2 - 1x10-7 mol / L and T3 - 1x10-9 mol / L melatonin. Posteriorly thediluting the samples were packaged in straws of 0.25 ml and cryopreserved. After thawing at 37°C for 30seconds, the samples were analyzed for Test thermoresistance slow (TTR) and acrosomal membrane integrity.For statistical analysis we used the Duncan test (α = 0.05) for mean comparison. The results demonstrated thatto the TTR at time 0 min, T2 showed 54.28% motility, and T1obtained lower values in the times 0 and 60 minutesfor force. Supplementation of 1x10-9mol / L of melatonin improved total motility.(AU)
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