Efeito da Insulina-N sobre a Integridade das Membranas Plasmáticas em Sêmen Fresco de Ovino, utilizado Gema de Ovo de Codorna

Barcelos, Wayllba AssunçãoPinto, Sâmara Cristine CostaLima, Talyta Luiza MirandaAbreu Junior, Giovani Santos deSouza, Fernando Andrade

This study aimed to evaluate the effect of insulin on the membrane integrity evaluated by the eosin testand nigrosine in ovine semen. Seven sheep were used, harvest were made with the aid of eletroejaculator. Theyolk-citrate extender was used for dilution of the ejaculate (25x106 sperm / mL) using quail's egg yolk. Eachejaculate was divided into four equal fractions, corresponding to treatments: control group (Gcont) using onlydilutive citrat gem; Group 50 (G50) plus dilutive 50UI/ml insulin type N; Group 100 (G100) plus dilutive 100IU/Ml Insulin type N; Group 200 (G200) plus dilutive 200 IU/Ml Insulin type N. After dilution, the semenremained in water - bath at 45°C to perform the eosin test and nigrosine for evaluation by time (0, 20, 40 and 60minutes) for membrane integrity. Thus, the use of insulin does not preserve the integrity of the membranesduring the incubation period of 45C, with a gradual decrease due to stress caused by time and temperature atwhich the semen was subjected.(AU)

1. ovine
2. ovino
3. semen
4. Insulina-N
5. sêmen
6. Insulin-N