Efeito da estimulação hormonal ovariana e polivinilpirrolidona sobre a maturação in vitro de oócitos de cabras Anglo Nubiana
Mudo, George Antonio MacielSilva, Ana Arlete de AmorimRibeiro, Laisa Gomes MedeirosSilva, Maria Naiara Pereira daLoiola, Maria Lilian GomesOliveira, Raphael Amorim deCordeiro, Mabel FreitasLopes Júnior, Edilson Soares
To evaluate the effect of hormonal stimulation treatments with PVP on the production and maturationof COCs, 27 goats received intravaginal devices (CIDR) for 10 days and 125 µg cloprostenol on day 8 oftreatment. The goats were distributed in the following groups: FSH-eCG, 300 IU of eCG and 70 mg pFSH, 36 hprior to the withdrawal of CIDR; FSH, receiving 180 mg (40/40; 35/35, 30 mg) at 12 h intervals; FSH-PVP10and FSH-PVP40 receiving 70 mg FSH dissolved in PVP 10,000 MW (24 hours before CIDR) and PVP MW40,000 (48 hours before CIDR), respectively. After follicular aspiration, COCs of grade I and II were submittedto IVM. Recovery rate from the FSH-PVP10 was higher (73.33%) than FSH-PVP40 (44.26%) and FSH-eCG(27.14%). COCs with grades I and II, were more frequently observed (P < 0.05) in FSH-PVP40 than FSH-eCG.FSH-PVP40 is an alternative to recovery and mature oocytes in vitro.(AU)
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