Comparação entre diferentes concentrações de gema de ovo na qualidade de espermatozoides epididimários caninos resfriados a 4°C
Medeiros, Hian Raphael Rodrigues DeSantos, José Fabson Pinheiro DosMorte, Roberta Ferreira Da BoaSilva, José Sergio DaSiqueira, Andressa Kathily De MacedoSouza, Wandson João Da Silva ESilva Neto, Jairo De Macedo Lins ECardoso, Rita De Cássia Soares
The epididymal spermatozoa recovering allow reproduction even with the death of a breeder. Using aproper extender is important and ACP-106c®extender presented good results for canine ejaculate. Egg yolk hasbeen used as a protective membrane against thermal shock, being necessary to determine optimal concentrationfor dog epididymal spermatozoa using ACP-106c®. Thirteen pairs of epididymis were used and spermatozoawere recovered by epididymal tail compression by glass blade. ACP-106® was used as extender and the sampleswere divided into 3 different concentrations of egg yolk (0, 10 or 20%), then analyzes of motility (%) and vigour(0-5) were made at times 0, 60, 120 and 180 minutes as well as after 24 hours. There was no difference in theevaluation of motility and vigour between the three egg yolk concentrations. The concentration of 20% egg yolkshowed higher number of functional membrane.(AU)
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