Caracterização física e estrutural de espermatozoides no ejaculado de Caprinos Azul, Canindé, Moxotó e Repartido
Silva, Jefferson Hallisson Lustosa daBarçante, Felipe Pereira da SilvaNascimento, Isolda Márcia Rocha doBranco, Yndyra Nayan Teixeira Carvalho CasteloLustosa, Micherlene da Silva CarneiroSousa Filho, Marcos Antônio Celestino deBarros, Dayse AndradeSouza, José Adalmir Torres de
This study aimed to evaluate physically and structurally ejaculates from locally adapted goats in therainy season. Semen pool formed by the four goats of each breed was evaluated for physical, diluted in Tris-eggyolk and frozen. After thawing, it was diluted in Tris and incubated with carboxyfluorescein diacetate andpropidium iodide, to evaluate the integrity of the plasma membrane and JC1 to assess mitochondrial activity.The percentage of cells with damaged membrane and mitochondrial activity in spermatozoa between breed werecompared, every hour for three hours. There was no significant difference (P > 0,05) in relation to thepercentage of cells with damaged plasma membrane, as well as between the incubation times. The Azul breedhad lower mitochondrial activity (P < 0,05). The evaluated semen of all breeds showed no significant damage tothe plasma membrane in the rainy season, but the Azul breed had lower mitochondrial activity in sperm cells.(AU)
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