Azul de toluidina como método para avaliação da integridade da cromatina de espermatozoides canino extraídos do tecido testicular fresco
Santos, José Fabson Pinheiro DosSiqueira, Andressa Kathily De MacêdoMedeiros, Hian Raphael Rodrigues DeMorte, Roberta Ferreira Da BoaMontanhas, Anne Karoline Batista DasSouza, Wandson João Da Silva ESilva Neto, Jairo De Macêdo Lins ECardoso, Rita De Cássia Soares
Conservation of gonadal tissue is important for maintenance of endangered wild species. However, thepreservation can lead to cell damage and loss. Thus, it is necessary testicular tissue evaluation before and afterstorage in order to determine damage. The evaluation of sperm chromatin integrity ensures a better assessmentof sperm quality. The toluidine blue is a simple and sensitive test to evaluate the sperm chromatin integrity fromseveral species, but there are not reports in the dog. The aim of this study was evaluating canine spermatozoaextracted from fresh testicular tissue of seven post-pubertal mongrel dogs by toluidine blue and spermmorphology. For statistical analysis, it was used normality test (Shapiro Wilk) and Wilcoxon test (P<0.05).Toluidine blue showed 83% condensed chromatin. Morphological analysis presented 69.34% normalspermatozoa. Toluidine blue was sensitive for the evaluation. However, it still needs some adjustments for amore accurate result for such specie.(AU)
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