Avaliação da maturação nuclear de oócitos bovinos submetidos a três diferentes sistemas de incubação

Campelo, Iana SalesTeixeira, Thiago Victor DamascenoSilva, Mirelly Mirna Alves de SousaCavalcanti, Camila MunizVieira, Luís AlbertoFigueiredo, José Ricardo deMoura, Arlindo de Alencar AraripeFreitas, Vicente José de Figueirêdo

This study aimed to evaluate three different incubation systems in nuclear maturation of bovine oocytes.Follicles of 2 to 8 mm in diameter were aspirated for obtaining cumulus-oocyte complexes (COCs). Thereafter,COCs were subjected to in vitro maturation (IVM) in TCM-199 medium supplemented at 38.5 °C for 23 h indifferent incubation systems: bench incubator with high oxygen (20% O2 in air), bench incubator with lowoxygen (5% O2) and portable incubator with low oxygen. After IVM, cumulus cells were removed and oocyteswere stained with Hoechst 33342. The slides were analyzed by a fluorescence microscope and the oocytes wereclassified into: degenerated (DG), metaphase I (MI) and metaphase II (MII). No difference (P > 0.05) wasobserved among the rates of DG, MI and MII for the different incubation systems. Therefore, the use of portableincubation system is a viable alternative for in vitro maturation of bovine oocytes.(AU)

1. low oxygen
2. portable incubator
3. in vitro maturation
4. oxigênio reduzido
5. maturação in vitro
6. incubadora portátil