Análise da integridade de membrana de espermatozoides extraídos de fragmentos frescos de testículos de cães

Siqueira, Andressa Kathily De MacêdoSantos, José Fabson Pinheiro DosSilva, Arivonaldo Vaniel DaMontanhas, Anne Karoline Batista DasMedeiros, Hian Raphael Rodrigues DeSilva Neto, Jairo De Macêdo Lins ECardoso, Rita De Cássia Soares

To evaluate testicular tissue before cryopreservation is important to quantify and qualify losses ofsperm during the freezing/thawing of testicular tissue. The aim of this study was to analyze the sperm plasmamembrane structural and functional integrity on post-pubertal dogs (n = 7) fresh testis. The sperm fromtesticular tissue were submitted to plasma membrane structural and functional integrity analysis by fluorescentmicroscopy using SYBR 14 and Propidium Iodide and the hiposmotic test, respectively. For statistical analysis,it was used normality test (Shapiro Wilk) and Wilcoxon test (R 3.3.1; P > 0.05). It was observed 94.9% and91.8% spermatozoa presenting structural and functional integrity, respectively. Thus, analysis of the membraneintegrity is an important tool to analyze spermatozoa extracted from testicles. Despite the fluorescence be apractical method, it has a high cost.(AU)

1. fluorescent probe
2. canino
3. canine
4. sonda fluorescente
5. hypoosmotic
6. hiposmótico