Topical effects of autogenous, allogenous and xenogenous blood serum on the healing of alkali burns in the cornea of dogs

T. J. Brunelli, AdrianaF. Campos, CarlaC. Talieri, lviaF.A.M. Vicenti, FelipeR.E. Moraes, JulietaL Laus, José

The topical effects of autogenous, allogenous and xenogenous blood serum on wound healing of alkali corneal ulcers weredetermined in 52 dogs divided into four groups (GI, Gil, Glll and GIV). The effects of autogenous serum were studied in Glanimais, those of allogenous serum in Gil, and those of xenogenous serum in Glll. GIV animais received 0.9% buffered salinesolution also by instillation. Events such as blepharospasm, secretion, conjunctival hyperemia and chemosis, corneal edema,neovascularization and pigmentation were evaluated macroscopically and statistically. The inflammatory response and evolutionof corneal wound healing were analyzed by light microscopy. NÓ significant clinicai or histopathological differences wereobserved between groups. Corneal perforations occurred in tive Gl animais at 4, 10, 11, 16 and 18 days of treatment, and in threeGil animais at 4, 6 and 7 days. In Glll, one animal presented corneal perforations at 7 days and in GIV, two animais presentedperforations at 8 days and one animal at 9 days of treatment. In the absence of perforations, the epithelium was found to bereconstituted, on average, at 15 days of treatment. Comparison of the groups showed no significant differences betweentreatments.