Polymorphisms and their haplotype combinations in the lysozyme gene associated with the production traits of a chinese native chicken breed

Yan, L. JFang, X. TLiu, YZhang, C. LChen, H

Animal lysozymes, which have been studied in many of invertebrate and vertebrate species, have been characterized and demonstrated to be immune-associated molecules, digestive enzymes and multifunctional molecules. The purpose of this study was to detect the connection between lysozyme-gene polymorphism and the production traits of a Chinese native chicken breed (Langshan chicken). Four single nucleotide mutation sites were identified: G345A, C1726T, G1836A, A1838G. By the linkage disequilibrium analysis, six haplotypes and 15haplotype combinations were depicted in the studied population. The statistical analysis demonstrated that the SNPs and the haplotype combinations are related to body weight at sixteen weeks of age in Langshan chickens (p 0.05), and those with combined haplotype Hap3-Hap6 (GA-TT-GG-AA) presented higher body weight. Our study demonstrated that the SNPs and their haplotype combinations in the lysozyme gene were associated with the chicken production traits, and that SNPs can be used as a molecular marker for chicken marker-assisted selection.(AU)

1. Lysozyme gene
2. Association
3. PCR-SSCP
4. Haplotype
5. Langshan chicken