In-vitro cytotoxicity of aflatoxin B1 to broiler lymphocytes of broiler chickens

Zimmermann, C. E. PMachado, A. KCadoná, F. CJaques, J. A. SSchlemmer, K. BLautert, CCruz, I. B. MZanette, R. ALeal, D. B. RSanturio, J. M

The aim of the present work was to study the in-vitro cytotoxic effects of different concentrations of aflatoxin B1 (AFB1) on broiler lymphocytes. Lymphocyte-rich mononuclear cells were separated by Ficoll-Histopaque density and cultured in 96-wellplates containing the evaluated AFB1 concentrations in 5% CO2 atmosphere at 39°C. Thereafter, MTT, PicoGreen, and reactive oxygen species assays were performed. Cell viability decreased in the presence of 10 µg/mL AFB1 at 48 h (p 0.05) and of 10 and 20 µg/mL AFB1 at 72 h (p 0.01 and p 0.001, respectively) when compared to the control (0 µg/mL). However, a dose-dependent increase in the cell-free DNA at 24 h was observed at 1, 10 and 20 µg/mL (p 0.001). ROS formation significantly increased at 24 h at all concentrations (p 0.001). The in-vitro results demonstrate that AFB1 is cytotoxic and causes biomolecular oxidative damage in broiler lymphocytes.(AU)

1. Reactive oxygen species
2. Aflatoxin B1
3. PicoGreen
4. Broilers
5. Lymphocytes