Importance of the association of molecular and immunological diagnosis in immunocompetent patient with Histoplasma capsulatum and Cryptoccocus neoformans infection: a case report

Dantas, Kátia CristinaFreitas, Roseli Santos deGarcia, Roberta Scholz PintoSilva, Marcos Vinícius daMuricy, Edna Cleide MendesKohara, Valdelene SayuriVicentini, Adriana Pardini

This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity against Histoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.(AU)

1. Nested PCR
2. Immunoblotting
3. Blood fluid
4. Fungal infections
5. Immunocompetent individuals