Identification of two novel cytolysins from the hydrozoan Olindias sambaquiensis (Cnidaria)

Haddad Junior, VidalZara, FernandoMarangoni, SergioToyama, Daniela de OliveiraSouza, Alex Jardelino Felizardo deOliveira, Simone Cristina Buzzo deToyama, Marcos Hikari

Although the hydrozoan Olindias sambaquiensis is the most common jellyfish associated with human envenomation in southeastern and southern Brazil, information about the composition of its venom is rare. Thus, the present study aimed to analyze pharmacological aspects of O. sambaquiensis venom as well as clinical manifestations observed in affected patients. Crude protein extracts were prepared from the tentacles of animals; peptides and proteins were sequenced and submitted to circular dichroism spectroscopy. Creatine kinase, cytotoxicity and hemolytic activity were evaluated by specific methods.(AU)

1. Myonecrosis
2. Cytotoxicity
3. Cytolysin
4. Olindias sambaquiensis
5. Hemolytic
6. Cnidaria venom