Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon

Casseb, Alexandre do Rosário; Nunes, Márcio Roberto Teixeira; Rodrigues, Sueli Guerreiro; Rosa, Elizabeth Salbe Travassos; Casseb, Lívia Medeiros Neves; Casseb, Samir Manssor Moraes; Silva, Sandro Patroca da; Rodrigues, Érika Dayane Leal; Vasconcelos, Pedro Fernando da Costa

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Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon

Casseb, Alexandre do RosárioNunes, Márcio Roberto TeixeiraRodrigues, Sueli GuerreiroRosa, Elizabeth Salbe TravassosCasseb, Lívia Medeiros NevesCasseb, Samir Manssor MoraesSilva, Sandro Patroca daRodrigues, Érika Dayane LealVasconcelos, Pedro Fernando da Costa

The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.(AU)