Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry

Yu, HaiLiu, Huiyan, YizhongDuan, ZhiguiWang, Xianchun

Numerous spider toxins are of interest as tools for neurophysiological research or as lead molecules for the development of pharmaceuticals and insecticides. Direct detection and identification of the interacting proteins of a spider toxin are helpful for its action-mechanism analysis and practical application. The present study employed a combinative strategy for the analysis of interacting proteins of huwentoxin-IV (HWTX-IV), a peptidic neurotoxin from the venom of the spider Selenocosmia huwena.(AU)

1. Far-western blotting
2. Huwentoxin-IV
3. Biotin labeling
4. Mass spectrometry
5. Interacting protein