Vipoxin specificity studied by gas chromatographic determination of enzymatic reaction products. Influence of Ca2+, Mg2+ and Sr2+

Atanasov, V.Bardarov, V.Aleksiev, B.Mitewa, M.

Gas chromatographic procedure with mass spectrometric detection was applied to quantitatively determine the enzymatic specificity and activity of vipoxin (a neurotoxin from the Vipera ammodytes meridionalis venom) as well as the influence of Ca2+, Mg2+ and Sr2+ on these properties.

1. Ca2+
2. Mg2+
3. Sr2+ influence
4. vipoxin
5. phospholipase A2, enzyme specificity