Detection of rabies virus from Artibeus lituratus Olfers, 1818, by the immunoperoxidase and RT/PCR techniques

C. Cardoso, T.gnerWagnerWagnerRahal, P.Pilz, D.H. Q. Silva, L.

A diagnostic capability that includes virus isolation and identification in cell cultures is highly desirable, even in countries where rabies is not endemic. The potential use of CER in the diagnosis of rabies infection has also been evaluated and this system can be used to study the processes of infection. In this study the challenge virus standard (CVS) and field rabies strain isolated from a frugivurous bat Artibeus lituratus Olfers, 1818, were propagated in CER monolayer, and 72h post-infection the cytophatic effect was observed.