Novel nonsense mutation in the katA gene of a catalase-negative Staphylococcus aureus strain

Lagos, JaimeAlarcón, PedroBenadof, DonaUlloa, SoledadFasce, RodrigoTognarelli, JavierAguayo, CarolinaAraya, PamelaParra, BárbaraOlivares, BertaHormazábal, Juan CarlosFernández, Jorge

We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme to just 283 amino acids. (AU)

1. Catalase-negative
2. Catalase gene
3. Staphylococcus aureus
4. katA