Quinolone resistance and ornithine decarboxylation activity in lactose-negative <italic>Escherichia coli</italic>
Gomig, FrancianeGalvão, Carolina WeigertFreitas, Denis Leandro deLabas, LarissaEtto, Rafael MazerEsmerino, Luiz AntonioLima, Marcelo Andrade deAppel, Marcia HelenaZanata, Silvio MarquesSteffens, Maria Berenice ReynaudNader, Helena BoncianiSilveira, Rafael Bertoni da
<p>Quinolones and fluoroquinolones are widely used to treat uropathogenic <italic>Escherichia coli</italic> infections. Bacterial resistance to these antimicrobials primarily involves mutations in <italic>gyrA</italic> and <italic>parC</italic> genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic <italic>E. coli</italic> strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic <italic>E. coli</italic> strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region <italic>gyrA</italic> and <italic>parC</italic> mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative <italic>E. coli</italic> isolates.</p>.
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