GPo1 <italic>alkB</italic> gene expression for improvement of the degradation of diesel oil by a bacterial consortium

Luo, QunHe, YingHou, Deng-YongZhang, Jian-GuoShen, Xian-Rong

<p>To facilitate the biodegradation of diesel oil, an oil biodegradation bacterial consortium was constructed. The alkane hydroxylase (<italic>alkB</italic>) gene of <italic>Pseudomonas putida</italic> GPo1 was constructed in a pCom8 expression vector, and the pCom8-GPo1 <italic>alkB</italic> plasmid was transformed into <italic>Escherichia coli</italic> DH5α. The AlkB protein was expressed by diesel oil induction and detected through SDS-polyacrylamide gel electrophoresis. The culture of the recombinant (pCom8-GPo1 <italic>alkB/E. coli</italic> DH5α) with the oil biodegradation bacterial consortium increased the degradation ratio of diesel oil at 24 h from 31% to 50%, and the facilitation rates were increased as the proportion of pCom8-GPo1 <italic>alkB</italic>/<italic>E. coli</italic> DH5α to the consortium increased. The results suggested that the expression of the GPo1 gene in <italic>E. coli</italic> DH5α could enhance the function of diesel oil degradation by the bacterial consortium.</p>.

1. Alkane hydroxylase
2. <italic>Escherichia coli</italic> DH5α
3. Diesel oil
4. Biodegradation
5. Expression