A new culture medium for recovering the agents of Cryptococcosis from environmental sources

Silva, Dulcilena de Matos Castro eSantos, Dayane C.S.Pukinskas, Sandra R.B.S.Oshida, Julia T.U.Oliveira, LidianeCarvallho, Anderson F.Melhem, Márcia S.C.

The isolation of Cryptococcosis agents from environmental samples may be difficult due to the presence of groups of fast-growing fungi. We propose a new culture medium based on a modification of Dichloran Rose-Bengal Chloramphenicol Medium (DRBCm) to detect colonies of Cryptococcus neoformans. Our results indicate that DRBCm is superior to the classical Bird Seed Agar in its ability to detect colonies of C. neoformans.

1. isolation techniques
2. DRBC
3. Cryptococcus