A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases

Angelini, Leticia Mara LimaSilva, Amanda Ribeiro Martins daRocco, Lucas de Freitas ColiMilagre, Cintia Duarte de Freitas

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

1. nitrilase
2. amidase
3. amidase inhibitor
4. high-throughput screening
5. nitrile hydratase