Whole cells in enantioselective reduction of benzyl acetoacetate

Ribeiro, Joyce BenzaquemRamos, Aline de SouzaLopes, Raquel de OliveiraSilva, Gabriela Veloso Vieira daSouza, Rodrigo Octavio Mendonça Alves de

The β-ketoester benzyl acetoacetate was enantioselectively reduced to benzyl (S)-3-hydroxybutanoate by seven microorganism species. The best result using free cells was obtained with the yeast Hansenula sp., which furnished 97% ee and 85% of conversion within 24 h. After immobilization in calcium alginate spheres, K.marxianus showed to be more stable after 2 cycles of reaction.

1. Immobilization
2. β-ketoester
3. Biotransformation
4. Kluyveromyces marxianus
5. Chiral reduction