Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Spositto, F.L.E.Campanerut, P.A.Z.Ghiraldi, L.D.Leite, C.Q.F.Hirata, M.H.Hirata, R.D.C.Siqueira, V.L.D.Cardoso, R. Fressatti

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.

1. tuberculosis
2. bovine tuberculosis
3. diagnosis
4. PCR-multiplex