An improved purification procedure for Leishmania RNA virus (LRV)

Souza, Marcos Michel deManzine, Livia ReginaSilva, Marcos Vinicius G. daBettini, JeffersonPortugal, Rodrigo VilaresCruz, Angela KayselArruda, EuricoThiemann, Otavio Henrique

Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.

1. Leishmania guyanensis
2. LRV
3. Virus isolation
4. Electron microscopy
5. Leishmania RNA virus