Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal, C.A.G.; Carvalho-Castro, G.A.; Cottorello, A.C.; Leite, R.C.; Figueiredo, H.C.P.

Comparative analysis of conventional PCR and real-time PCR to diagnose shrimp WSD

Leal, C.A.G.Carvalho-Castro, G.A.Cottorello, A.C.Leite, R.C.Figueiredo, H.C.P.

The aims of this study were to standard and optimize a qPCR protocol with FAM-BHQ1 probe, and to compare its sensitivity against TaqMan qPCR and PCR methods to diagnose shrimp WSD. The FAM-BHQ1 qPCR presented higher clinical sensitivity and showed to be a robust alternative to detect WSSV in clinical samples.