Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Mendonça Moraes, PaulaMartins Perin, LuanaSilva Júnior, AbelardoAugusto Nero, Luís

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.

1. API
2. PCR
3. 16sDNA
4. identification
5. lactic acid bacteria
6. Biolog