Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Mendonça Moraes, PaulaMartins Perin, LuanaSilva Júnior, AbelardoAugusto Nero, Luís

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.

1. PCR
2. lactic acid bacteria
3. 16sDNA
4. API
5. Biolog
6. identification