Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method

Garcia Monte, LeonardoJorge, SérgioPaulo Mesquita Luiz, JoãoSinnott, FrancineKömmling Seixas, FabianaAntonio Guimarães Aleixo, JoséErnesto Samartino, LuisRochedo Conceição, FabricioPinho Hartleben, Cláudia

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.

1. Canine leptospirosis
2. LipL32
3. IMS-PCR