Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method

Monte, Leonardo GarciaJorge, SérgioLuiz, João Paulo MesquitaSinnott, FrancineSeixas, Fabiana KõmmlingAleixo, José Antonio GuimarãesSamartino, Luis ErnestoConceição, Fabricio RochedoHartleben, Cláudia Pinho

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.

1. LipL32
2. IMS-PCR
3. Canine leptospirosis