A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Qiu, ShanlianChen, JichenLin, SiLin, Xinjian

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

1. Silver staining
2. Backed gel
3. DNA