Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Santos, Carla RFranciscatto, Laura GBarcellos, Regina BAlmeida, Sabrina E MRossetti, Maria Lucia R

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

1. HPV
2. FTA elute
3. Cervical cancer
4. Molecular detection