Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

Santos, Carla R.Franciscatto, Laura G.Barcellos, Regina B.Almeida, Sabrina E. MRossetti, Maria Lucia R.

This study aimed to evaluate the use of the FTA elute cardTM impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.

1. HPV
2. FTA elute
3. molecular detection
4. cervical cancer