16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Navarro-Noya, YendiHernández-Rodríguez, CésarC Zenteno, JuanBuentello-Volante, BeatrizE Cancino-Díaz, MarioJan-Roblero, JanetC Cancino-Díaz, Juan

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

1. Staphylococcus epidermidis
2. PCR
3. endophthalmitis
4. DGGE