Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Vaucher, Rodrigo de AlmeidaDezen, DiogenesSimonetti, Amauri BragaSpilki, Fernando RosadoRoehe, Paulo Michel

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.

1. phylogenetic analysis
2. Bovine parainfluenza virus type 3
3. bPIV-3