An in-house multiplex pcr method to detect of putative virulence factors in Aeromonas species

Guadalupe Aguilera-Arreola, Ma.Aidee Carmona Martínez, AlmaCastro-Escarpulli, Graciela

A pentaplex PCR was developed and optimised to detect the genes that encode the five most important putative virulence factors in Aeromonas isolates. It seems to be more efficient than previously reported techniques and promises to be a powerful tool for more accurate risk assessments and for monitoring pathogenic strains.

1. virulence potential
2. pathogenesis
3. Aeromonas
4. pentaplex-PCR