Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests

Cargnelutti, Juliana FelipettoBrum, Mário Celso SperottoWeiblen, RudiFlores, Eduardo Furtado

West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.

1. West Nile virus
2. M/E glycoproteins
3. WNV
4. stable expression
5. diagnostic