Detection of Bartonella henselae in defibrinated sheep blood used for culture media supplementation

Drummond, Marina RovaniPitassi, Luiza H. ULania, Bruno GSantos, Silvio R. C. dosGilioli, RovilsonVelho, Paulo E. N. F

Bartonella henselae was detected in defibrinated sheep blood employed in supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure a sterile culture medium for Bartonella spp. isolation, since infected blood samples used in preparation could lead to false-positive results.

1. Sheep-Blood Contamination
2. Culture Media
3. Polymerase Chain Reaction
4. False Positive Reactions
5. Bartonella