Differentiation of Bovine coronavirus (BCoV) genotypes by a restriction enzyme assay

Pinheiro de Souza, SibeleMiyuki Asano, KarenLucas Sandri, ThaisaNunes de Barros, IracemaJosé Richtzenhain, LeonardoEduardo Brandão, Paulo

This article reports the use of the GsuI restriction enzyme to differentiate genotypes of Bovine Coronavirus (BCoV), based on an 18-nucleotide deletion of S1-coding region found in one of the two genotypes. It was concluded that this assay can be used as a rapid tool for BCoV genotypes differentiation.

1. restriction enzyme
2. genotypes
3. Bovine coronavirus
4. gene S