Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

von Groll, AndreaMartin, AnandiPortaels, FrançoiseEduardo Almeida da Silva, PedroCarlos Palomino, Juan

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.

1. Growth kinetics
2. Mycobacterium tuberculosis
3. Fitness
4. Resazurin