A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis

Kumar, GavishShankar, HariBisht, DeepaSharma, PrashantSinghal, NeeljaKatoch, V.M.Joshi, Beenu

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.

1. SDS-TCA procedure
2. sre filtrate proteins
3. Two-dimensional gel electrophoresis
4. M. tuberculosis