Glass bead transformation method for gram-positive bacteria

Rattanachaikunsopon, PongsakPhumkhachorn, Parichat

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.

1. transformation
2. glass bead
3. Gram-positive bacteria