Protease obtention using Bacillus subtilis 3411 and maranth seed meal medium at different aeration rates

Pastor, Maria DeliaLorda, Graciela SusanaBalati, Antonio

The influence of the addition of Amaranthus cruenthus seed meal to the medium, as nutrient and growth factor, on protease production by Bacillus subtilis 3411 was studied. Tests were carried out in a rotary shaker and in mechanically stirred fermenters. The influence of aeration was also evaluated. The addition of amaranth in a concentration of 20 g/L resulted in 400 per cente increase in protease production. Aeration up to 750 r.p.m. and 1 L/L.min had a favorable effect.