Protein profile analysis by SDS-Page of "Mycoplasma gallisepticum" strains S6(208) and F-K810 grown in Hayflick's and Frey's media

Ferraz, Priscila NMenezes, Cláudio C. PDanelli, Maria das Graças MLizeu, Jackeline de O. PNascimento, Elmiro R. doLucchesi, Daniela M

The "Mycoplasma gallisepticum" strains [wild-type S6(208) and vaccine-type F-K810] grown in Frey's and hayflick's media were analysed by SDS-PAGE. No visual changes in the protein profiles of these strains were observed regardless of the media composition used, although the polyacrylamide gel electrophoretograms showed minor differences do exist when densitometer traces of the gel are compared. Both strains were easily differentiated on SDS-PAGE analysis by a peptide band p75, that is specific for MG F-K810 strain, used as vaccine.