Preservação do sistema genital de cabras para a avaliação em microscopia de luz

Salmito-Vanderley, C.S.B.Neves, M.M.Santana, C.V.Marques Jr., A.P.Vasconcelos, A.C.Nunes, J.E.S.

The preservation of the goat genital organs with a fixed solution of paraformaldehyde 10% and a saturated solution of Bouin during 4, 8, 12 and 24 hours of fixation, included in paraffin and stained with hematoxylin-eosin was evaluated. The Bouin solution with four hours of fixation showed to be the best protocol of fixation for the ovary, oviduct, uterus and vagina, resulting in little tissue retraction and better cellular integration when compared to the other fixing times (8, 12 and 24 hours). The fixation with paraformaldehyde showed significant alteration in the tissue architecture of the oviduct and vagina. Althouh paraformaldehyde is not the most adequate solution for the goat genital system preservation, its use can be considered in the absence of Bouin solution.

1. microscopia de luz
2. Goat genital
3. solução fixadora
4. genitália
5. fixing solution
6. light microscopy
7. Cabra