The effects of cyanocobalamin supplementation during the thawing of frozen boar semen on spermatozoa, in vitro fertilization, and embryonic development
Mello, A RHyde, A MElsea, L EWhitaker, B D
The objective of this study was to assess the in vitro fertilization (IVF) of pig oocytes using frozen - thawed boar sperm in media supplemented with cyanocobalamin . Frozen semen pellets were thawed and incubated for 1 h in fe rtilization media containing cyanocobalamin (0, 0.5, 1.0 or 2.0 μ m ) and evaluated for forward progressive motility, viability , and embryo cleavage . Forward progressive motility of the 0.5 and 1 .0 μ m cyanocobalamin supplements was higher (P < 0.05) than the 0 and 2 .0 μ m cyanocobalamin supplements. Membrane v iability of sperm supplemented with 0.5 μ m cyanocobalamin was higher (P < 0.05) than all other groups. Oocytes were matured and fertilized with frozen - thawed boar semen that was previously incubated for 1 h in fertilization media containing cyanocobalamin (0 or 0.5 μ m ; 100 oocytes/treatment). Fertilization characteristics were evaluated 12 h after IVF of oocytes and embryo development was analyzed at 48 h and 144 h post - IVF. There were no significant differences between treatment groups when evaluating fertilization , polyspermic penetration or male pronucleus development. Embryos derived from oocytes fertilized with 0.5 μ m cyanocobalamin supplemented sperm had a higher percentage (P < 0.05) of cleaved embryos compared to those without cyanocobalamin supplementation at 48 h after IVF. There were no significant differences in the percent ages of embryos reaching the blastocyst stage by 144 h afte r IVF between treatment groups. The results of this study suggest that there are positive effects of 0.5 μ m cyanocobalamin supplementation during the incubation of frozen - thawed boar semen on early development of IVF derived pig embryos.(AU)
Texto completo