Regulation of superoxide dismutase by prostaglandin F2α in the bovine corpus luteum
Vu, H VDam, T VAcosta, T J
The interaction between prostaglandin F2 α (PGF) and r eactive o xygen species (ROS) is crucial for regulating the life span of the corp us luteum (CL) . The local accumulation of ROS is mainly controlled by copper/zinc superoxide dismutase (SOD1). Thus, PGF may induce luteolysis by decreas ing the expression of SOD and its bioactivity. Here, we examined whether SOD1 is involved in the luteol ytic action of PGF in the bovine corpus luteum (CL). SOD activity gradually increased from the early to late luteal stage and then decreased to the lowest level at the regressed luteal stage. SOD1 protein expression and SOD activity increased at 2 h but de creased at 24 h after administration of a luteolytic dose of PGF . In addition , PGF and H 2 O 2 increased SOD1 protein expression and SOD activity at 2 h but suppressed it at 24 h in cultured luteal cells . Furthermore, H 2 O 2 increased PGF production by luteal c ells in a dose - and time - dependent manner. PGF , in turn, induced ROS production. T hese results indicate that PGF via interaction with ROS regulate s bovine luteal SOD 1 in a biphasic manner with an initial increase at 2 h followed by a decrease at 24 h . The down regulation of SOD1 during structural luteolysis may enhance ROS production and luteal cell death to ensure the regression of the bovine C .(AU)
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