A fatal pneumonia due to coinfection of Pseudomonas putida and Staphylococcus pseudintermedius in a laboratory beagle dog
Hong, MinWei, LipingChen, YanQin, YongchangWang, XinZhang, YaqunChang, YanLi, Hua
Background: Pseudomonas putida (P. putida) is widely distributed in the environment, and sometimes caused nosocomialinfections in human beings, but no case of infection has been reported in beagle dogs. Staphylococcus pseudintermedius(S. pseudintermedius) is a natural cutaneous bacterium in dogs and occasionally causes purulent infections of the skin yetrarely causes pneumonia. Both bacteria are opportunistic pathogens. Dogs, even well-controlled laboratory beagle dogs,maybe infected by the bacterium in certain conditions like this report. In order to provide information and give suggestionto veterinarians involved in dogs study, a complete profile of the coinfection was drawn in this report.Case: It is presented a case of an 8-month-old beagle dog, weighing 6 kg that suffered from coinfection of P. putida andS. pseudintermedius during a treatment of chemotherapy. The animal was confirmed as normal by appearance, physicalexamination and laboratory tests before arrival according to the applicable guidelines. After 14-day acclimation period, theanimal was administrated with a tyrosinase inhibitor once daily via oral gavage. From Day 8, coughing, decreased activity, hyporeflexia, squinting, shortness of breath (abdominal breathing), and discharge around the nose as well as cracklesin the lung and rapid heart rate were noted. Since the poor conditions progressed quickly and have not been improved bytreatment of ceftriaxone and dexamethasone. On Day 9, the animal was euthanized for humanitarian reasons. To define thepathogen, hilar lymph node and thoracic swab were collected for bacteria isolation and purification in special mediums,and at last characterized by Gram staining and 16s rRNA gene sequence analysis and positive PCR-restriction fragmentlength polymorphism. In clinical pathological examination, an increase in WBC, neutrophils, lymphocytes, monocytes...(AU)
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