Relationship of circulating tumor necrosis factor alpha (TNF-alfa) and insulin secretion and resistance in euglycaemic dogs

Lakić, IvanaBelić, BranislavaCincović, MarkoPotkonjak, AleksandarTrailović, DragišaKovačević, Zorana

Background: Insulin resistance is a state that is characterized with reduced sensitivity of peripheral tissues to insulin. Itcan be related with increased level of tumor necrosis factor alpha (TNF-α) in dogs. Insulin resistance can be evaluated byhomeostasis model assessment (HOMA-IR, HOMA-β). The aim of this study was to determine correlation of circulatingTNF-α level with insulin production and insulin resistance indexes in euglycaemic dogs.Materials, Methods & Results: Seventy dogs of normal body score were included in this study. After blood sampling levelsof glucose, insulin and TNF-α were determined and indexes HOMA-IR and HOMA-β were calculated. Three groups inaccordance to TNF-α levels were formed: the first-TNF-α 0-2.0 pg/mL, the second-TNF-α below median (2.1-17.0 pg/mL)and the third-TNF-α above median (17.1-51.8 pg/mL). Differences in insulin and glucose levels, HOMA-IR and HOMA-βwere determined in all three groups. ANOVA and posthock LSD analyses were used. Correlation between HOMA-IR andHOMA-β was determined. Linear regression between HOMA-β/HOMA-IR ratio and glucose concentration was calculated. SPSS statistical program was used (IBM). Highest insulin level was detected in the second group and the lowest wasdetected in the third group. The lowest glucose level was detected in the first group. The highest value of HOMA-β indexwas noted in the first group and it decreases with TNF-α increase. The highest HOMA-IR value was detected in the secondgroup and the lowest was in the third group. Positive correlation was noted between HOMA-IR and HOMA-β. Significantlinear correlation was noted between glucose levels in function of HOMA-β/HOMA-IR...(AU)

1. dogs
2. insulin resistance
3. HOMA
4. TNF-α