Microbiome and antimicrobial resistance in members of the Enterobacteriaceae family from vaginal and preputial mucous isolates of stray dogs
Rey, Laisa Marina RosaGuaitolini, Carlos Renato de FreitasFazoli, Kawany Gabrieli ZanettiSilva, Lucas Lima daFendrigo, Túlio TozziSantos, Isabela Carvalho dosZaniolo, Melissa MarchiMartins, Lisiane de AlmeidaGonçalves, Daniela Dib
Background: Contact between humans and pets, mainly dogs and cats, has been increasing in recent years, which may result in the spread of infectious agents to new hosts and even to the environment, causing emergencies of national and international interest. The aim of this work was to understand the phenotypic profile of bacteria of the Enterobacteriaceae family of vaginal and preputial mucous of stray dogs from a border region. Materials, Methods & Results: Swab samples from the vaginal and preputial mucosa of stray dogs from two border regions were collected for later bacterial isolation, biochemical identification of bacterial isolates, susceptibility tests to different antimicrobials, and determination of the bacterial resistance index. Samples were collected from 70 animals, was possible to isolate 88 samples, of which 36 (40.9%) presented isolates of Gram-negative bacteria, with Escherichia coli being the most prevalent species (44.8%), followed by Obesumbacterium proteus in eight (27.5%); Enterobacter aerogenes and Enterobacter cloacae in two (6.8%); and Erwinia herbicola, Koserella trabulsii, Proteus mirabilis, and Serratia rubidaea (3.4%) from one isolate. The most resistant antimicrobials Clindamycin (100%), Metronidazole (100%), Oxacillin (100%), and Penicillin (100%) were tested against the vaginal and preputial samples and when the multidrug resistance index of the isolates was analyzed, all were classified as presenting a public health risk. Discussion: The results of this work suggest that stray dogs may be considered potential reservoirs of resistant pathogenic microorganisms, enabling future health problems due to the close coexistence of tutors with their dogs. It is known that the microorganisms that inhabit a certain environment or a specific part of the body are collectively called microbiomes. More specifically, some of them are bacteria that inhabit the reproductive mucous...(AU)
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